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Isocitrate Assay Kit (EFIC-100)

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Product details

Management number 220390891 Release Date 2026/05/03 List Price US$179.60 Model Number 220390891
Category

ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing alpha-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. BioAssay Systems’ isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at ex/em = 530/585 nm, is proportional to the isocitrate concentration in the sample. This product is for Research Use only and is not intended for diagnostic purposes.

  • For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.
  • Method: Fluorometric assay (530/585 nm). A spectrophotometer or plate reader is required, but not provided.
  • Samples: Food, beverage, biological samples. Species: All. Detects 0.6 uM
  • Procedure: 10 min. Size: 100 tests per kit
Item Weight 1 pounds
Manufacturer BioAssay Systems
Product Dimensions 5.5 x 4 x 2 inches
Is Discontinued By Manufacturer No

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